On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis

Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope. Such steps are time-consuming and can lead to loss of vesicles when they are transferred to an observation chamber. To overcome these issues, we present an integrated microfluidic chip which combines GUV formation, trapping, and multiple separate experiments in the same device. First, we optimized the buffer conditions to maximize both the yield and the subsequent trapping of the vesicles in micro-posts. Captured GUVs were monodisperse with specific size of 18 ± 4 µm in diameter. Next, we introduce a two-layer design with integrated valves which allows fast solution exchange in less than 20 s and on separate sub-populations of the trapped vesicles. We demonstrate that multiple experiments can be performed in a single chip with both membrane transport and permeabilization assays. In conclusion, we have developed a versatile all-in-one microfluidic chip with capabilities to produce and perform multiple experiments on a single batch of vesicles using low sample volumes. We expect this device will be highly advantageous for bottom-up synthetic biology where rapid encapsulation and visualization is required for enzymatic reactions

On the Importance of Frictional Energy Dissipation in the Prevention of Undesirable Self-Excited Vibrations in Gas Foil Bearing Rotor Systems

In this contribution, a nonlinear and fully coupled fluid–structure–rotor interaction model of a gas foil bearing rotor system is presented. Aiming at the reduction of undesirable self-excited vibrations, many common bearing designs feature a compliant and slightly movable multi-part foil structure inside the lubrication gap. The present paper discusses the general impact of frictional energy dissipation within the foil structure by adding equivalent viscous damping to the widespread simple elastic foundation model. For the computational analysis, the PDEs describing the fluid pressure distribution and the foil structure deformation field are spatially discretized using finite difference schemes. After suitable nondimensionalization of the resulting system of nonlinear ODEs, a corresponding state-space representation is deduced. Using numerical simulation tools, the stability of equilibrium points and the occurrence of self-excited vibrations are addressed and possible bifurcation scenarios are discussed. Summing up all results, frictional energy dissipation proves to be of crucial importance with regard to the reduction or prevention of undesirable self-excited vibrations in gas foil bearing rotor systems

Shape of a liquid front upon dewetting

We examine the profile of a liquid front of a film that is dewetting a solid substrate. Since volume is conserved, the material that once covered the substrate is accumulated in a rim close to the three phase contact line. Theoretically, such a profile of a Newtonian liquid resembles an exponentially decaying harmonic oscillation that relaxes into the prepared film thickness. For the first time, we were able to observe this behavior experimentally. A non-Newtonian liquid - a polymer melt - however, behaves differently. Here, viscoelastic properties come into play. We will demonstrate that by analyzing the shape of the rim profile. On a nm scale, we gain access to the rheology of a non-Newtonian liquid.Comment: 4 pages, 4 figure

Isolated development of inner (wall) caries like lesions in a bacterial-based in vitro model

The study conducted in a bacterial-based in vitro caries model aimed to determine whether typical inner secondary caries lesions can be detected at cavity walls of restorations with selected gap widths when the development of outer lesions is inhibited. Sixty bovine tooth specimens were randomly assigned to the following groups: test group 50 (TG50; gap, 50μm), test group 100 (TG100; gap, 100μm), test group 250 (TG250; gap, 250μm) and a control group (CG; gap, 250μm). The outer tooth surface of the test group specimens was covered with an acid-resistant varnish to inhibit the development of an outer caries lesion. After incubation in the caries model, the area of demineralization at the cavity wall was determined by confocal laser scanning microscopy. All test group specimens demonstrated only wall lesions. The CG specimens developed outer and wall lesions. The TG250 specimens showed significantly less wall lesion area compared to the CG (p < 0.05). In the test groups, a statistically significant increase (p < 0.05) in lesion area could be detected in enamel between TG50 and TG250 and in dentine between TG50 and TG100. In conclusion, the inner wall lesions of secondary caries can develop without the presence of outer lesions and therefore can be regarded as an entity on their own. The extent of independently developed wall lesions increased with gap width in the present settin

Комплекс геофизических исследований скважин с целью определения коллекторских свойств палеозойского резервуара на Герасимовском месторождении (Томская область)

Изучение геологического строения месторождения и анализ ранее проведенных геофизических исследований в скважинах Герасимовского месторождения Томской области позволил построить физико-геологическую модель пласта М нефтегазоносного горизонта зоны контакта. В проектном разделе представлено обоснование заложения глубокой скважины и определен комплекс геофизических методов исследования для решения геологических задач. Рассмотрена методика проведения работ и используемая аппаратура. Рассчитана стоимость затрат на проведение проектных работ и даны рекомендации по минимизации воздействия их на человека и окружающую среду.The study of the geological structure of the field and the analysis of previously conducted geophysical studies in the wells of the Gerasimovskoye field of the Tomsk region made it possible to build a physical and geological model of the reservoir M of the oil and gas horizon of the contact zone. The project section presents the rationale for laying a deep well and defines a set of geophysical research methods to solve geological problems. The technique of work and the equipment used are considered. The cost of design work was calculated and recommendations were made to minimize their impact on humans and the environment

Cardiac cell modelling: Observations from the heart of the cardiac physiome project

In this manuscript we review the state of cardiac cell modelling in the context of international initiatives such as the IUPS Physiome and Virtual Physiological Human Projects, which aim to integrate computational models across scales and physics. In particular we focus on the relationship between experimental data and model parameterisation across a range of model types and cellular physiological systems. Finally, in the context of parameter identification and model reuse within the Cardiac Physiome, we suggest some future priority areas for this field

Pseudomonas aeruginosa AES-1 exhibits increased virulence gene expression during chronic infection of cystic fibrosis lung

Pseudomonas aeruginosa, the leading cause of morbidity and mortality in people with cystic fibrosis (CF), adapts for survival in the CF lung through both mutation and gene expression changes. Frequent clonal strains such as the Australian Epidemic Strain-1 (AES-1), have increased ability to establish infection in the CF lung and to superimpose and replace infrequent clonal strains. Little is known about the factors underpinning these properties. Analysis has been hampered by lack of expression array templates containing CF-strain specific genes. We sequenced the genome of an acute infection AES-1 isolate from a CF infant (AES-1R) and constructed a non-redundant micro-array (PANarray) comprising AES-1R and seven other sequenced P. aeruginosa genomes. The unclosed AES-1R genome comprised 6.254Mbp and contained 6957 putative genes, including 338 not found in the other seven genomes. The PANarray contained 12,543 gene probe spots; comprising 12,147 P. aeruginosa gene probes, 326 quality-control probes and 70 probes for non-P. aeruginosa genes, including phage and plant genes. We grew AES-1R and its isogenic pair AES-1M, taken from the same patient 10.5 years later and not eradicated in the intervening period, in our validated artificial sputum medium (ASMDM) and used the PANarray to compare gene expression of both in duplicate. 675 genes were differentially expressed between the isogenic pairs, including upregulation of alginate, biofilm, persistence genes and virulence-related genes such as dihydroorotase, uridylate kinase and cardiolipin synthase, in AES-1M. Non-PAO1 genes upregulated in AES-1M included pathogenesis-related (PAGI-5) genes present in strains PACS2 and PA7, and numerous phage genes. Elucidation of these genes' roles could lead to targeted treatment strategies for chronically infected CF patients. © 2011 Naughton et al